FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012893

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012893
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2870693
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAAAT29550.07.47668651
CCTCAGG11350.00148882327.13743541
CACACAG18002.4623441E-66.95539471
CTCACGG16252.2829037E-46.3448311
CACGAAT14350.00253522986.15849731
CCTACGG16953.934605E-46.0828031
CAACGAA25051.8405754E-65.87986951
CTTTTAT64000.05.7535441
CCACGAT24507.890898E-65.71127271
CTTTAAA51100.05.6206831
CCCGTGA16000.0084949795.5234021
CTCGAAG20155.947456E-45.4822851
CCTGAAT27358.663967E-65.3854011
CGAAAAT42405.020411E-105.38444851
CTCTAAA19300.0020518975.34215071
CCTCGAC19350.00211971375.32834671
CTTAAAC18100.00562788455.2894461
CAACGAT26703.3233962E-55.2406811
CTCCGAA22504.5845314E-45.23700331
CTTCGAG19700.00265415665.23368021
Produced by FastQC (version 0.11.5)