FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012894

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012894
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1120288
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT4450.024.1702699
CACACGT4650.023.131727
ACACGTC5607.2759576E-1219.2067328
AGAGCAC7857.6397555E-1114.6196263
AGCACAC7404.8930815E-1014.5354725
GAGCACA8001.03682396E-1014.3416664
GCACACG8051.1277734E-1014.2525876
GAAGAGC14156.9399175E-78.1469091
CCCACGC8050.0049712918.0551921
AAGAGCA15803.8932158E-67.26484632
AGTCACT3653.1430558E-56.30928720-24
CATAAAT16050.00175458745.83576251
Produced by FastQC (version 0.11.5)