FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012903

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012903
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences907495
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT4059.144753E-615.8922589
CACACGT4351.7980885E-514.7970547
ACACGTC4804.5483313E-513.4090928
AGCACAC4958.829259E-411.5586415
GCACACG5702.2625341E-411.2924886
CTCCAAC7001.2536593E-410.2707781
GAGCACA5800.00330092669.8663334
AGAGCAC6300.0065277299.0832913
TTGTGCA8750.00123269778.1731619
ACATTGC9900.00414112637.22415076
TTCCGAA14656.397903E-46.3460549
CCGAAAA17500.00501239775.34080461
Produced by FastQC (version 0.11.5)