FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012910

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012910
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1031771
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCTAT5901.8235656E-512.4097491
CTCCGTT7751.5484511E-712.2816491
CTCACAT4950.00973520210.3539921
CTCAACG4950.00973520210.3539921
CCCAAAT5850.002922582410.0126521
CTTGAAT8658.5064494E-59.3108981
CTCTCAT7800.00323490828.4481751
ATCACGA8000.00406565248.2369711
GTATGAA12205.058365E-57.8018671
CTTCCGT13151.5211017E-57.79502151
CTCTGTT10900.00102924757.38892361
CTTATAT12951.0606657E-47.35002141
CTTATTT16103.8011094E-67.27627561
CTTTATT20404.9705704E-87.17818831
CTTTGAA15751.6373795E-46.5082241
CAATAAT17701.1009726E-46.20487451
ATCACAT13100.00710321736.1480361
CTTTTAC16803.7736518E-46.101461
CTTCTGT17154.91836E-45.976941
CACAAAA18652.2519102E-45.88880831
Produced by FastQC (version 0.11.5)