FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012911

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012911
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences832731
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTATC7853.79771E-49.1995062
ATCTCCC6600.0088479138.75241856
CTCAATG7750.00306723278.4976491
GATATAG7850.00388360128.2785615
CTTCAAT11853.5407895E-58.0275491
CTTCAGA9450.00239218637.64100036
CTCTTTT18304.5831985E-77.19746261
CTTCTTT26455.2750693E-117.1929281
CTCCTTT15201.0371895E-46.73972941
CAATGAT12200.0034017086.5976741
CATCAAT16202.3758585E-46.3236961
CTTCCAT14300.00261344746.1404931
TTCAATT13200.0088575386.01801062
CTTTTTA15800.0079192325.5575341
TTTTAAA22104.6753068E-45.2283172
Produced by FastQC (version 0.11.5)