FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3552793
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACGT7550.00251865068.6861891
CCTAAAC7650.00283753488.5726431
CCCCAAT17452.2360291E-77.51641561
CTTCAAT47050.07.27900361
CTCACGA15201.4212363E-57.1908691
CTCTGAT26454.474714E-106.88728481
CTTAGAT10600.0067181326.87428951
CTTAATT20906.270948E-76.62431571
CTTTAAT39050.06.53101541
CTCGAAT14655.1118614E-46.4660561
CTCACAG19207.9646325E-66.45180751
CACCAAT28352.1154847E-96.4257031
CCCATTT33405.0931703E-116.32681661
CTCAGAT20804.0762334E-66.30583861
CACCCGT18703.738026E-56.234651
CCCACCT20051.5566897E-56.17828941
CTCTCAC13200.0080898256.07228951
CTTCGAT37452.3646862E-116.0317531
CTCACAT13450.0098202175.95942161
CTTTTAT62050.05.87167361
Produced by FastQC (version 0.11.5)