FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012921

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012921
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2377594
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAAGA13503.4742698E-99.603081
CCCAAGA12901.6903869E-89.4914161
CTCTAAC6150.00506532059.3688591
CTCAAGG18301.2732926E-119.0520841
CTCTAAT9302.2388225E-48.5188621
CTCAGGG7650.00315236118.4733071
CTCATTT24851.8189894E-127.82544761
CTTCGAA14105.800017E-67.66203171
CTCAGAC8550.0085665917.5813791
CTTAAAC12701.02345264E-47.37244271
CTTAAAT21954.638423E-97.2187161
CTTTGAT37550.06.9050111
CTTCTAT27451.8735591E-106.82186031
CTCGATT11650.00248026856.8004641
CCCAAAT17151.24416365E-56.7193561
CCACACT10850.0094141056.63807341
CTCAGCT11950.00323946296.6297411
CTCAACT10900.0098403336.60762361
CTTACGA12000.0033843786.6021171
CTCAAAG24353.6732672E-86.50722071
Produced by FastQC (version 0.11.5)