FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012926

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012926
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1381752
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCACAG8650.0083046997.6073111
CCCAGGA9700.0027367687.53759961
CTTTTAT26304.7293724E-117.22807071
CCCAAAA14103.0732973E-46.7410731
GTTCATT14303.6497094E-46.64679151
CTTTTGT21651.0830936E-66.41653351
CTTTGAA20451.9978217E-56.0779961
TTTAATC13200.008848956.01923472
AATCTTG15000.0051510555.77867465
CTTTAAT15500.00647538165.66049431
CTTTGAT22508.572033E-55.52422241
AGATTTT28654.8374022E-65.2945974
TCAGATT17900.00619479835.2458142
TCCGTTT20850.00121589535.19646172