FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1329486
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCCTT8808.850904E-610.0367591
TCCCTTC10801.2352545E-48.0221362
CCTCGAT12254.9895993E-57.81092071
CTTATTC11602.2615922E-47.61409331
CTCAATG10950.0083373466.7217271
CCTCGAA11150.0099265136.6011571
CTTGTAT13450.00122866716.56680151
CATCTAT12650.0046784586.4002531
CCCAAAA12650.0046784586.4002531
ATAATTC17451.0993158E-46.20600375
CTTCAAT20301.6098234E-56.16378971
CTTTGTG14450.00275491626.1123521
CTCCGAA13250.0075733916.11042931
ACGATTT15600.00121862716.0163763
CTTTCCC16200.00152012795.90640541
CTTCAAG16650.00210842145.74677371
CATCACC22105.9049533E-55.66176181
CTTCCTT22855.737076E-45.1538141
CTCCTTT23357.7794585E-45.0434541
CTTCTTT41003.6603524E-85.02653931
Produced by FastQC (version 0.11.5)