FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences943218
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTAC4300.003477623511.8895561
CTCCACT5109.5106155E-411.4566031
TCACAGT5750.00287281310.0332853
CTCAATT5900.00320080379.9031651
CCCTTGT7000.00123722619.3903221
CTCTATT6300.0055008579.2743931
CAATGAG8054.364322E-49.0727761
CACAAAT9803.4621706E-58.9431641
CCCACAT6600.0080602698.852831
CCCATTT8850.00112021528.2526391
CCCAGAT8950.00125192188.1604291
CTCATTT10154.9335574E-47.9152141
CTTTAAT11502.2355186E-47.62113141
CTCAAAG11500.00186440556.98603731
TTGATAT19101.1874308E-66.79610543
CCTTGAC10750.00751405356.79403161
TCCGTTT13852.9175682E-46.76920372
ATTTGAT15301.15887495E-46.68301871
CTTTTCT22503.098794E-76.49207451
CCCAGCT13050.00700370646.15627771
Produced by FastQC (version 0.11.5)