FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2067497
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT9500.00245802697.6210661
CCCAAAG17201.2020344E-56.73489571
CTCCGAT16203.786757E-56.7037161
CCCGCAT18504.9335904E-66.6529851
GATTTAG10900.0097193796.61602835
CTCACGG14454.6742405E-46.5135071
CCCCATT12350.0043280586.44859461
CTCGAAA18603.7932405E-56.22796871
CTCGCAG12950.00709031576.1498181
CTCCGCG17851.4451673E-46.08404451
CTTCGAT22803.1123109E-66.03334431
CCTCGGT14700.00401360735.91021441
CCACAAT17356.599345E-45.84208541
CTTTGAA32307.041308E-95.82787471
CAACGAT17607.9210906E-45.7591011
CTTCAAT29702.6777707E-75.60674431
GTCAGGA19655.3320406E-45.52672771
CTTAAAT15850.0092358085.4813981
AAGCCTG20057.3722034E-45.3951165
CTTGAAT20358.531476E-45.3366191