FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012956

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012956
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5090596
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTCGT19401.638382E-77.15612171
CTATGAT54750.06.80631261
CTTACGA23651.5501428E-86.7970021
CTCGGAC19451.2970686E-66.76205541
CTCAGAT26102.2810127E-96.7188761
CTCATGT25157.4705895E-96.68214231
CTTAAAC28854.274625E-106.5849641
CTCCCAG36650.06.579091
CTTCGCT37000.06.51685521
CTCGAAA33304.3655746E-116.363261
CTCACGC26001.0084477E-76.18265771
CATAGAT45250.06.13607841
CTCTTGT37051.6370905E-116.11363271
CTTAAAT51900.06.05378441
CTCGAAT18201.6646562E-46.0220691
CTTTAAT74050.06.01908731
CTCCTAT30359.0203685E-96.01876161
CTCAATT34105.18412E-105.99969961
CCCAATT22009.697405E-65.97827241
TGAGTCT16250.0096982625.966705145
Produced by FastQC (version 0.11.5)