FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012958

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012958
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1071463
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGAA6802.845809E-813.9802791
CTCCCCT4302.2350794E-413.6051381
CTTCGAG6757.301731E-510.8337211
CTTTAAT16950.010.7857841
CTTCGAT10708.710231E-810.2515351
TTCGAGA5850.00325667659.8827342
CTTACTT6758.770341E-49.7503491
CCCACTT6100.00417374969.5905071
CTTAAAG8709.181949E-59.246021
CCTCAAT8608.3295885E-48.5032111
CTTCTTA10507.66169E-58.3574421
CTTCCTT21152.1827873E-118.2981691
CTTGTTT15904.3750333E-88.2785981
CTTTGAA19753.9835868E-108.1458611
CCCATTT11753.213296E-58.0907141
CTTCCCG15401.9894833E-67.5976751
CTATAAT11853.1144673E-47.4053281
CCTTTTA10800.00107232917.3605784
CTTATAT13351.5675332E-47.12104131
TTCCCGA13502.0798964E-46.95909172
Produced by FastQC (version 0.11.5)