FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1112249
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT4502.0306197E-615.3455827
CACGTCT4705.182568E-513.2245099
ACACGTC6003.9405953E-511.5102218
GAGCACA7001.5799142E-510.8515194
GCACACG7101.8483719E-510.698686
AGCACAC7150.00249998838.6922525
AGAGCAC7150.00249998838.6922523
CAACGAA9956.8993715E-47.67148071
CTCCAAC9100.0024418757.6254981
CACAGAT9150.0025766727.5838291
AACGAAA11906.272217E-46.9666732
CTTGTTC12550.0010923466.63509461
AACATTT14300.0049914685.7948353
CAGATTT15150.0094444495.46971233
ATTCAAA16800.00470798235.36962131
CTTCAAA17850.0095695.05376151
Produced by FastQC (version 0.11.5)