FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012962

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012962
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1047383
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAAAT8103.6097863E-610.8193061
CCCAGCT8054.3686642E-49.0720891
CCTCAAT8406.6794927E-48.6940851
CTCTCCC8000.00416068738.2159111
GAACCGC9250.00196207947.7971485
TCAAGGG8450.0076088247.68180662
CTTCAAG15552.3473676E-67.5143731
CTTAAAT8800.0097783777.46901041
CTTAAAA12809.4789895E-57.4171411
CTTCATT16951.0860113E-67.3245741
CTTCAAT16254.5358793E-67.1906771
CTCCAAA11300.00155038787.1091461
CTAAAAT10450.00571812276.9885471
CTTTGTT14958.574885E-56.8389591
CTTTCAT19301.1456395E-66.81111761
CTCTTTT24251.7859202E-76.3242751
TCAAATC13700.00190112586.3173982
CTTTATT19155.099808E-56.10174941
CTTTGAT17607.079556E-45.80923031
CTTATTT15300.00567704445.7278681
Produced by FastQC (version 0.11.5)