FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012977

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012977
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences430967
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCACTT1801.5006267E-424.2084311
CCCAGTT3304.9213914E-415.4053651
CAACGAC3050.005181081614.28694251
CTTCGAA3609.518116E-414.1215851
CTCAAAT4250.003318466511.9618121
CTTCGGA4700.007028791610.8165321
CCTGCTT6808.413482E-510.680191
CTTCAAA8355.5246073E-610.4371671
GGAAAAC8200.0054493877.97106841
CTCTTTT11051.4975824E-47.886911
CTTTATT9900.00355609627.33588841
CTTTCTT13150.0080117496.07511951
CTTCTTT17155.430509E-45.9285951
Produced by FastQC (version 0.11.5)