FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012978

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012978
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1073850
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCAGT6302.4895962E-713.49992751
CCTCGCT6700.00109121689.5204721
CTCAAAC8751.3741924E-48.9099521
GGGGGGG2250.00213264496.89904885-89
GTTTGAT11450.00244495726.80891561
CTTTGAT16100.002214865.72279551
CTTCGGC20151.6403454E-45.62776141
AAATCCG20953.0457505E-45.3891644
CATCAAT17250.00500988545.34127571
CTTCCTT17900.0077269255.14731841
Produced by FastQC (version 0.11.5)