FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012979

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012979
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences979814
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGAA4750.00783038910.6625661
TCCCATG5750.002906478710.0192617
GTTTGAT11854.4901753E-79.1586251
CTAATTT13803.9943643E-67.8644711
ATCCGCT9650.0030166597.4625336
CTTGAAA16705.812733E-56.4987841
CTTATAT16603.7681777E-46.10207131
ACTTCAA15400.00107500066.0790577
Produced by FastQC (version 0.11.5)