FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012980

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012980
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1819630
Sequences flagged as poor quality0
Sequence length100-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT6050.024.5748847
CACGTCT8250.018.0215829
ACACGTC8950.015.821028
GAGCACA11700.012.7075254
AGCACAC13250.012.2893125
GCACACG14150.012.0079946
CCTTAGC4400.00510771811.2939021
AGAGCAC13957.2759576E-1210.6582173
AGTCACT5602.0167136E-86.06879220-24
ATCAGAT21601.1464144E-55.9158541
AAGAGCA27801.0972144E-75.86005352
GAAGAGC33452.420893E-85.5179261
GTTTGAA17400.00544756375.3038691
CTCCGAG18150.0089224865.0847011
Produced by FastQC (version 0.11.5)