FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012995

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012995
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2423367
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAAGT8557.601086E-59.4086241
CTCAGGT8400.0063784297.83542871
CTCATTT22801.1641532E-107.69796471
CTATGAT24805.475158E-96.78227951
CTCGTAT12050.003012446.67582851
CCCAAAA22252.4851397E-76.5735431
CTCCAAA26301.843182E-86.39545771
CTCCTGT22251.728813E-66.2448661
CTCAAGA14800.0038712085.92951351
CTTCGAT22601.4740377E-55.82456641
CCCACAT15100.00483933325.8117081
CCCGCTT17757.7677664E-45.76805261
CTTCACT23052.023173E-55.71085451
ATTTGCG21605.602023E-55.68213376
CCCATGA18250.00110590875.61002351
CCTCAAT18450.00126971545.549211
CTTCAAT43854.5474735E-115.5035621
CTTTTAT53800.05.4372241
CTCAAAT20407.709428E-45.3772551
CTCTTGA17750.00485759975.35604861
Produced by FastQC (version 0.11.5)