FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005012998

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005012998
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences775550
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAAGT3100.005519114.1534921
CGAATCT4200.003186314412.0289474
CTCTGAT5007.9698965E-411.7002211
CCCAGTT5058.6634606E-411.5843771
CTCAATT5300.001298035411.0379451
CTCCAAT6950.00114522849.4696041
TAAAACC6350.00647038879.0927483
CCACGAA6600.0079761528.8638041
CTTCCAA8356.207443E-48.757651
CAACCGT6750.0095871488.666831
CTTGTAT7800.00326963268.437661
CATCATT11903.761509E-57.98859551
CTTTAAA9550.00234241397.6572131
CTCCGTT8700.0087222517.56479841
CTCAAAG10350.00513875867.06535051
CTTCTTT20351.8423974E-56.1088381
CTTTTAT13300.0084111266.04804661
CTTTCTT17808.030957E-45.7515131
Produced by FastQC (version 0.11.5)