FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences752831
Sequences flagged as poor quality0
Sequence length100-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGGG2700.00241899916.0215789
GTTCGCT3850.001630647713.1513651
GTTAGTG3300.00916376713.1094357
TCTAGGA1254.921751E-410.3841950-54
TAGCATC6000.0040894149.6135858
AGGGGTG2250.00169791899.6129479
GAGTATA7500.00260007998.6539524
AAGGATG9653.2821004E-48.2205074
GGGTGGG1600.00467750158.11092410-14
GGTGGGT2004.8413745E-47.93068110-14
CTAGGAG1650.00615548767.866811350-54
CGAAGAT8500.0078111247.6587361
TTCTAGG1700.0080418597.635433750-54
ACACCCC2150.0085061986.708489435-39
CTTTGAT13650.00176408636.3589021
TGGGTCA2550.0062984766.22072115-19
CTTGGAC3150.00138848375.95205830-34
ACTAATC4201.87014E-45.5777273135-139
GGGGTGG3400.00345477355.513307610-14
TTTGATT17150.0038078915.4665392
Produced by FastQC (version 0.11.5)