FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013010

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013010
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4035607
Sequences flagged as poor quality0
Sequence length100-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGCTT25458.076313E-96.65687751
CCCAAAT22753.2650132E-76.4755871
CCTTAAT24101.3591671E-76.4184891
CTCACGA16050.00134956675.9662141
CCCAGAT44007.2759576E-125.6918941
CTTCAAA73800.05.58936171
CTCAAAA53000.05.55923031
CTCACTT20758.796617E-45.3248051
CTCTCAA19750.00273400615.22145371
CCCACAT27555.5639786E-55.0799861
Produced by FastQC (version 0.11.5)