FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013011

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013011
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences869923
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAAAG6403.2941134E-612.4918021
CTATGTT6153.938789E-410.6360361
CCCAGAA8104.8744443E-48.9727871
CTTTACT7300.00189612478.9604961
TTTAAGC6650.0095876148.667027
CTCAAAT7750.00326157628.4402091
CTTATTG7700.00332503748.422253
CTTCGAT8250.00572856667.9286811
CTCAAAA10608.2665496E-47.54222061
CTTTTAT16759.701871E-77.37643531
CTTCAAG10200.0047340317.1254491
CTTTATT14807.996709E-56.8750951
CTGATAT11700.00235399886.83312231
GAACATC11850.0029462096.6888382
CTTTAAA11000.0098310676.6072341
CTCCTTT17157.883733E-56.35681441
CTTCTGG13100.0076639946.1028651
CTTTGAT14400.0030488056.05663161
ATTTGAA15950.00146589855.92372751
TTTAATT14650.0040665785.9022822
Produced by FastQC (version 0.11.5)