FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences837549
Sequences flagged as poor quality0
Sequence length100-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT3203.1832315E-922.9731319
CACACGT4004.1494786E-818.3785067
ACACGTC4501.5920523E-716.3364498
GAGCACA6608.909665E-712.1525294
AGAGCAC6155.3775584E-611.955643
CGTTCAG9600.00794390210.639236145
GCACACG8201.2024271E-59.7801356
AGCACAC7605.6016346E-59.6728975
AGGATTA6200.0099912288.6233455
AAGAGCA11105.297845E-68.4331532
GAAGAGC10300.0014433567.1569381
AAAGATT10500.00181210727.00468062
AGTCACG2603.4198363E-46.68325220-24
ACGTCTG4252.3309334E-55.66097210-14
CAGTCAC3809.942821E-45.276251320-24
CTCGTAT3400.0084106995.110966735-39
Produced by FastQC (version 0.11.5)