FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences439342
Sequences flagged as poor quality0
Sequence length100-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATTAC2500.001393904817.3958871
CACAAAT4251.2683688E-515.3493121
CCTTGTT3558.691139E-414.2923961
ACCTGTT6100.006376599410.957989145
CTCCAAA5900.00340246979.8281841
CAACAAA6500.00755592768.9209671
GCACTAT1656.229452E-48.75619135-39
CTCCATC6650.0091086068.7197431
TTTTGCG6650.0093934918.6869928
CTTCATT8800.00113886668.236691
TTTGATT10608.81073E-47.49526072
CAAAGAA13151.444902E-47.1656061
Produced by FastQC (version 0.11.5)