FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013051

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013051
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1034576
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT4257.9569327E-716.7873579
CACACGT4351.0145977E-616.4014427
ACACGTC4401.1431803E-616.2150638
TCACGAG3407.075759E-414.6889388
GCACACG5105.3058884E-613.9887896
AGCACAC5206.485934E-613.7197745
GAGCACA5057.4912925E-512.7145624
AGAGCAC5158.9994304E-512.4676783
GTCAGGT7650.00335418718.4144211
GAAGAGC11650.00266540686.75320531
GTATAAA12600.0060481856.2440351
GTTCCAT15450.0079620935.5551521
CTTCAAA18350.0092804295.06699471
Produced by FastQC (version 0.11.5)