FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013054

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013054
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498748
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTCAT3503.5342964E-922.7612651
ACCCCTT2800.003020079815.4956978
CTTAAAT4150.002836425612.2157941
CTTTCAT8957.186827E-912.13781
AAGTTTG4550.0056998811.1262335
CTTTGAA7552.4920193E-49.5923451
CTTTGAT8105.040693E-48.9410131
CAAAGAG6650.0091748818.7124461
TATAATT9703.3563317E-48.2021182
CCTGCAT8000.0044779298.1474981
CTTCATT8200.00559141257.9487791
CTTTTAC8650.00902125057.53525831
ATAATTT12607.1205944E-46.88766773
CTCTTTT10950.0097191856.61391
Produced by FastQC (version 0.11.5)