FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013068

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013068
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences740570
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCCT2650.003698917315.03228951
CACACGT4402.5876598E-614.9904027
AGCACAC5701.7631828E-713.8858455
CACGTCT5009.718096E-613.1915539
GAGCACA6658.1690814E-812.8974834
GCACACG6204.9046685E-712.7660186
ACACGTC5351.9489627E-512.3285548
AGAGCAC5402.1381622E-512.2177013
GTCACAG4350.007712222610.6838681
CATGAGG7559.874828E-68.053659145
CTTTGTG8750.00272442777.5400674
AAGAGCA8950.0033940577.3725692
GAAGAGC9450.0054270737.0256741
GAATCTC7251.0049007E-76.908819140-144
AATCTCG5806.102796E-56.477018140-144
AGTCACC5606.353042E-56.0112333130-134
CCATGAG9659.124415E-85.6485305135-139
GTTCTTC17050.0093722565.06219341
Produced by FastQC (version 0.11.5)