FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013072

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013072
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1887453
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGAT8750.0097606457.47116331
CTTCTAT18856.278082E-66.55075451
GTCAGGT14950.0046688465.8303391
CTCCGAC15450.00672207265.6416551
CCCATGA15800.0086054415.51668171
CAACGAG17800.0057544955.27918155
AATATTC29457.893617E-65.15439035
ATTCCCG19650.00324450555.1500186
CAACAAT22706.3058775E-45.119741
CTTTGAT27055.1722425E-55.10199551
GCCAGAT21400.00159649625.091331
Produced by FastQC (version 0.11.5)