FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences740956
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACTAG400.00260697267.2534145
CTCAGAG2100.009761238517.4768221
TGTAACC4659.762334E-817.0522387
GTGTAAC2850.003472500215.1756756
CTCCCAT5156.8897694E-512.8276481
TCAACTT6100.00469619369.45373
CTCCGTT6450.00640299789.1042051
CTCAAAA8557.5657445E-48.5851061
TTTTGGA10951.5857186E-58.5579727
CTTTGTT9702.8674965E-48.3240121
ATTTTGG9000.00150471418.0093846
ATCAAGG9950.00337334987.3771511
TTGCTGA10750.00105201527.37309749
CTTTGAT10050.00371933667.30374671
CTCCACC11150.00127373047.24151751
GATTTCA13953.2006766E-46.71754845
ATGATTT16250.00201921445.76675653
TTTCCGG19450.00294715385.18859868
AAAAGAT18800.0090853395.0757151
Produced by FastQC (version 0.11.5)