FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013087

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013087
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences601252
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAAGA4901.616645E-716.3124431
CTCAAAT5255.912676E-613.8408621
CTTCGAA4402.8621027E-413.2117311
ACTTAAT4050.0094612313.0448885145
CTCATTT6303.8513095E-511.5340511
CTCCAAT5752.1148351E-411.3735771
CTTATTG5300.001367802710.9682291
CCCATTT5400.001598625610.7651151
TTCGAAA5950.00372297139.7228642
CTTTGTT9903.99111E-69.5418051
CATGAAT6950.00121269519.4097951
CTTTTAA7954.0474496E-49.1401911
CTTCAAT11151.7982411E-58.4720961
CTTTGAT9502.5518995E-48.4137871
CTTGAAA11251.8357647E-47.7508821
TCAATAT12155.6081288E-57.73599243
CTTTATT11753.032717E-47.42105771
CTTCAAA12003.863252E-47.2664521
CTTCCAT10100.00430521287.1945071
CAAAGAT10800.00865300656.69460873
Produced by FastQC (version 0.11.5)