FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013088

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013088
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences621080
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCAC2000.008454272517.9180329
CCCCAAG3300.00927478413.0850231
CTTGTCT5150.001166419611.1794371
CAATTGA4550.005913972411.0719421
CTTCGCT5400.001732447410.661871
CCTCGAT6406.22158E-410.1204471
CTTCAAT9352.176197E-610.0061941
CTTCTAT7251.7667774E-49.9265691
AGTTTGC6150.00526056539.3239556
CCACATT6250.0058111339.2118561
GTAGAAA6300.0063968429.1048882
CCGATAT7700.00347435618.3806353
TCAGATT8550.0088979377.54747252
CAGATTT11300.0018797266.97974253
TTTGCGG10350.00624072746.9248448
AATTTGT10400.00653390176.8921055
TTTTTCG13500.00172421556.37136846
CAAAAAC12850.00694328736.1606521
Produced by FastQC (version 0.11.5)