FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013098

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013098
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489565
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGAT3558.6225226E-414.3079181
CCTCATT4151.7634948E-413.9877741
TTGTAGC3250.00811249313.3521666
GTCATGA4200.003058159712.0935971
CTCAAGC4300.00364698111.8123511
ATCCTGT5551.5407619E-411.7667441
CTTAAAA6505.3692755E-511.1633211
CTTCAAC6050.00415112369.594921
CAAAGAG6150.004752319.4389051
CTCTTTT10658.8237866E-47.49462371
CTTTCTT11100.00137000977.19078731
Produced by FastQC (version 0.11.5)