FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013109

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013109
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3610468
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGAAC17703.319401E-77.34083751
GGGGTGC12150.00376629436.5352591
CCTCGAA19759.472911E-55.84789041
CCCACGA15250.006239965.68012331
CCTGGTT19505.010045E-45.5526851
CTCCGAT29006.1304327E-65.22718241
CTTGAAT39001.7838829E-85.18250561
CCTTAAT22807.3312334E-45.06560661
CCAGACT20150.0045165125.01532841
Produced by FastQC (version 0.11.5)