FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013114

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013114
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1440299
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA15453.426976E-98.8971171
CCGATCT11503.0352803E-68.7901037
TCCGATC12155.7150522E-57.72638033
CTTTAAA26053.7107384E-106.9431481
CCTCCAG13800.00199396146.2910961
GCTCTTC15650.00123519036.0097051
CTTTTGG18352.1273308E-45.9139731
TTCCGAT21455.0150273E-55.7230912
CAACTTT16650.00258770565.6487621
CTTTGAT26055.0248364E-65.5545181
CGAAGAT15900.0096321265.46019651
GTTTTAT27852.7998503E-65.4552951
CTTCTGG17300.00407156775.43652531
CTTTTAA28003.0897263E-65.426071
CTTAAAA19250.0024797565.2616441
CACCATT17950.00628111335.23965931
CTTCTAT18100.00692361645.19623661
CTTCAAT22305.1872834E-45.19085931
CTTTTCG18200.0073841265.16768551
CTTTCAC18200.0073841265.16768551
Produced by FastQC (version 0.11.5)