FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2465220
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTAG4150.002589603212.3682071
CTCAAGT9502.1096057E-610.0340571
CCCCCTG8500.00692473867.76390741
CTTCGAA17953.921741E-87.76150471
CTCCAAT23001.3460522E-107.6513871
CCCAAAT16506.67138E-77.5547791
CCCAAAC12708.197522E-57.505791
CTTTAAT34300.07.48222351
CCCACAG14254.3385393E-57.20393751
CCCTCGC12807.314053E-46.87429331
CCCCAAG11850.00245683696.80661341
CTTAAAC13008.7256276E-46.7685351
CCCAACT12050.00292938346.69364071
CACCAAT24152.1866072E-86.67978241
CTCAAAC17901.7953957E-56.5542611
CTTTGAT39700.06.2797911
CTCAAAA29404.12183E-96.2351861
CTCCGAT19456.044234E-56.0319421
CCCAAAG19556.511185E-56.0010881
CCCAATT14700.00348432275.9857791
Produced by FastQC (version 0.11.5)