FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013120

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013120
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2450045
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAAGG9402.4248258E-59.2192611
CTTCAAT38350.08.6623391
CTCAATT15704.3806722E-88.2797181
CCCCTAC8000.004606578.1244751
CTCAAGT8900.00132592358.114331
CCCCAAT15703.8540747E-77.8197341
CTCCGCT18951.6525519E-87.62190441
GTTAATC9450.00248542827.6125852
CTCAAAC17252.1401502E-77.5357441
CTCAGAC8650.0092777837.5139641
CTCAAAT23302.4920155E-107.43871741
CGTTAAT9850.00359037387.3317311
CTCCAGT16104.6808673E-67.1768991
CCTAAAT10250.00529019777.04561471
CCCAAGA12758.3198026E-46.79694561
CTCTTCT26805.175025E-96.46724271
CTCCCTT18202.8654204E-56.3487951
CTCATCT19801.4718875E-56.2004961
AGTTAAT12900.00699350566.15808531
CTTAAAT22352.3141456E-66.13929941