FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1486006
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGAT7953.2248667E-510.1728881
CTCACAA6300.00521129659.3361561
CTCAAAT11803.3666474E-68.7229771
CTCAGAT8759.3800295E-48.402541
CTCAACG7950.00370276958.3232721
CCCAGAT13602.2547887E-57.5684651
TCTCATT14357.196206E-67.5455612
CTCAAGA8800.0092145657.51931951
CTCTCAT8800.0092145657.51931951
CTCCGCT13802.7366628E-57.45877741
CTTTAAT20909.235009E-97.3874011
CTCACCG10100.00384791437.2794291
CCCTCCG10250.004442347.1729011
CCCACAA10300.0046577777.13808061
TATCGAA11750.00265856766.7553259
CTCCTCC22051.9110303E-76.6686831
CTCAGAA11050.0091996226.6535961
CCCAAAA17005.9663013E-56.48725561
CCCAGAA17156.7372646E-56.4305161
CTCAATG12600.0045416426.4186071
Produced by FastQC (version 0.11.5)