FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013130

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013130
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2106857
Sequences flagged as poor quality0
Sequence length100-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAAAT11251.6597187E-79.81020451
CCCCAAT7400.00192172948.9484981
CTCAAGG17001.4053512E-88.223261
CCCCTGT9100.00137343128.0853341
CTCCGAT16406.681512E-88.0754741
CTTAGAT8300.0054236317.97817851
CCCACAA12456.137244E-57.682691
CTCCCAT15702.430359E-67.49824571
CCCAGAC8850.009631997.48235941
CTCAGCT13201.26666E-47.2461741
CTAAAGG10400.00508209837.0746671
CCCCAAA10750.0070052986.8443291
CTCAGGA14253.2411606E-46.71224551
CTCTGAA16855.227895E-56.549841
CTTTGAG22201.4982015E-66.29709051
CTTCATT36609.094947E-126.23189261
GAAACTA14050.00251107876.16357232
ATCAGAT37852.5465852E-116.02608351
CAACGGA15950.00126825585.9968341
CTCACCG16000.00131677375.97809361
Produced by FastQC (version 0.11.5)