FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1148707
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGGT4908.2988513E-411.64485551
CTCTGAT6507.801371E-49.8757331
CTTTAAC8004.8200243E-59.8071521
CTCTGAA7100.0017485449.0411641
GGGGGGG1750.00129207378.13601420-24
CCCAGCT9653.7006513E-48.13028051
CGCCTAT7900.0045970068.1256031
CCTCGAT12501.0972264E-57.98837141
CTTGAAA17002.435263E-87.9715891
CTCTACG8200.006425337.82832531
CTCGTCA8350.00755788537.6876971
CTCACCG10800.0012330537.26455641
CTTTACG12256.068287E-46.98691271
GAACGAT12050.00414536666.47469575
CTTTAAA14706.891012E-46.30762961
CTTGAAT13850.00243554086.17976051
CTTTTGC21157.5442076E-66.0701911
CTTTTCC31957.687049E-95.8042041
CCTTCAT14800.0051054895.7830871
ATTCCAT18703.335524E-45.72123581
Produced by FastQC (version 0.11.5)