FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences768918
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT3150.034.2508779
ACACGTC3450.031.272548
CACACGT4050.028.4155457
GCACACG4700.026.0228616
AGCACAC4750.025.7656965
AGAGCAC4500.023.997463
GAGCACA4650.023.2233494
GAAGAGC9951.6370905E-1113.0526321
AAGAGCA11101.6771082E-810.3793072
AAACCAG6500.0079998698.8606015
ACTAATC3102.796476E-67.428957530-34
CTAATCT3102.796476E-67.428957530-34
CCACTAA3255.6630233E-67.086082530-34
CGTCTGA5102.2904087E-57.0737311
CACTAAT3502.2728873E-66.9911830-34
ACCACTA4003.6274287E-76.83696230-34
ACGTCTG4756.952281E-65.75452210-14
CACCACT5301.2386827E-65.70320125-29
TCTCGTA4305.4567325E-55.692272735-39
AATCTCG4407.7328325E-55.56290335-39
Produced by FastQC (version 0.11.5)