FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013152

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013152
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1755887
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGTT6505.3460048E-511.1718441
CCCCCAT7450.0023013438.7725221
CTTAGAA7550.0025971578.6563291
CTTAACA8450.0071633167.7343541
CTCTGAT13151.7003333E-57.7310861
CCCAGCT11402.1630793E-47.64389321
CTCATAC10200.0047797377.11931231
CTTTACT16405.6718236E-67.0845841
CTCCTTT35200.07.0141411
AGACGAT16808.113695E-66.91590361
CTCAAAT15852.6778729E-56.872271
CCCATAT15051.0093541E-46.75506831
CCCTCTT12959.32223E-46.7289871
CCTCTCT10800.0083159526.7237951
CTATCGT11950.00297097636.68440871
CTCAAAA24002.339766E-86.65655661
CTTCAAG19651.6958038E-66.6519381
CTTATGG13150.00110930126.6266451
CTCCGGT22102.4950168E-76.57167241
CCTCTGT13350.00131605646.52736951
Produced by FastQC (version 0.11.5)