FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013155

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013155
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1008281
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC5950.019.241188
CACACGT5453.7587597E-815.7548197
CACGTCT6051.3522185E-714.1923579
AGCACAC7106.664777E-813.1012795
GCACACG7351.0473741E-712.6556586
GAGCACA8254.6929927E-711.27559954
GTCACGT4750.00811756510.6109781
AGAGCAC8457.631592E-610.1618963
CACTGAT7200.00182157559.0003831
CAAAAAT16003.4310462E-56.7502881
CCAGAAT12950.00102528826.6720991
CCACAAA11950.00324433796.62789731
AAGAGCA14505.5900007E-46.41733742
CTTCAAG18253.106477E-56.31259731
GAAGAGC16102.704163E-46.26113651
CCAGCTT15150.0059542895.7032131
ATAAGAA17550.0054841085.30023248
Produced by FastQC (version 0.11.5)