FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences866985
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCAAT11602.8538852E-68.8289581
CTTAATT6650.0084601598.80050851
CTCAAAT8406.564799E-48.7088361
GGATGCC10105.2594556E-47.86799346
CCCATTT9950.00348844757.35218331
CCCAAAA10950.00109005527.3488261
AGATATA10350.0057845626.97995044
CCTCTTT14604.6616147E-46.5137321
CTTTTAT17358.456256E-56.32457261
GATGCCG15259.1927696E-46.15837577
CTTCTTT28701.6831109E-86.11742641
CAAAAAG22752.5010595E-66.1095831
TCAATTT14200.00278926086.10499863
CTTTAAT14500.00306277336.05414251
CTTCATC16400.00189115765.79881051
CTTCAAA16400.00189115765.79881051
CTTTATT17908.587605E-45.72155951
Produced by FastQC (version 0.11.5)