FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences692982
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT3800.030.2679487
ACACGTC4100.028.053228
GAGCACA4950.027.3384444
CACGTCT4250.027.0631079
AGAGCAC5000.024.3585533
GCACACG4750.024.214366
AGCACAC6100.021.073735
AAGAGCA9500.014.2509392
GAAGAGC8453.8198777E-1113.6896571
CTCGTAT4352.6995287E-511.6422415145
ACTCATT5950.00644505449.0968438
TCGCTTA3909.1393304E-86.945472230-34
AGTCGCT4151.9397812E-66.203315325-29
CAGTCGC4408.0508653E-76.158794425-29
CAAAAAT13300.00261666656.13945671
CACAGTC5258.474126E-85.935904525-29
GTCGCTT4753.1686886E-65.702598630-34
AGTCACA5101.8381779E-65.58253120-24
AAAAAAA24000.05.526101660-64
GTCACAG5755.228103E-75.4230320-24
Produced by FastQC (version 0.11.5)