FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1818914
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAAAT14551.1713928E-78.4224591
CCCGAAT10900.00121579117.2747681
CTCCAGG10700.0081598026.737041
TTATAGC12450.00517178476.33930976
CTCCAAG13250.009397375.9845261
TGATGTA19907.133664E-45.40825134
GTTGAAA26303.6845642E-55.2077581
CTGAAAC18150.00746254065.16320851
ATTAAAT21100.0014641285.1246211
CTTGACG20150.0045893775.00847961