FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1137260
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAAAT10801.0242065E-58.860821
CAACCGT8658.2630676E-48.510171
CTCAAAT10456.708065E-58.4531641
CTCAACA9150.00144142898.0451331
CCCATCT8350.00569468747.93433241
CACAAGA9350.00178390387.8730451
CTCCGCT8600.007418447.7036821
CTTAATA8850.009582697.4860651
CTTCCCA11200.0012972817.22984461
CTTTAAT17701.4329164E-56.65427971
CTTATAT14350.00254388226.15578841
CTTGATT14500.00285810726.09210731
CACAAAA17254.946991E-45.97438571
GGCAAGA15300.00519937325.7735661
CTTCAAA23051.8154997E-55.74851751
ATTCAAT18058.828367E-45.7095931
CTCTTTG16000.00852373155.52097271
CTTGTTG16150.0094448635.4696941
CTTTTGA20206.171728E-45.46630951
ATCAAAG21953.239821E-45.3658651
Produced by FastQC (version 0.11.5)