FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences910219
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAAAT4553.0969968E-513.9683581
CTTAGAA4956.8335416E-512.83960151
CTCACGA6658.941925E-510.619221
GCCGATC5350.001931817510.5242732
CTTGTAT8106.156427E-59.5900741
TTTCGTC7603.5625175E-49.2586338
CAACGAT9956.1321134E-69.2264471
CCCTGCC7050.00179446249.0150391
CGATCTT6250.00698455479.0087784
TTTTCGT10006.7906185E-58.4438727
CCACAAC7700.0039896558.2540291
CTCAAAG10651.3566387E-47.95693641
CTTCGAA8000.0056274977.94450431
ATTTTGG8000.00580837767.91656546
CTTCTGG16007.408871E-77.5031431
CTCCATC14851.5857804E-57.13311241
CTTTATT12156.1876315E-46.9745991
CTCCAGA10350.0072110396.82297661
CTCACCG10400.00755496146.79017451
CTTCGAT10550.0086751886.6936311
Produced by FastQC (version 0.11.5)