FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005013193

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005013193
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences928232
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGAGT3000.0048109214.4499261
CTCAATT5700.002630795110.14031
CCCCCCC1150.00293263910.00988580-84
CTACTAT6000.00402233429.6332851
CTTTCGA6050.00430757749.5536711
CCCAAGA6350.0064166719.1023171
CCTATAT7350.00212957248.8468941
CTTAAAT7500.00255803768.6699561
CTCATAT7650.00306045088.4999561
TCTTGCT9250.0020096997.7779517
CTTGAAT10507.961274E-47.56900931
CTTCAAT14851.1502576E-57.2979431
ATTTTGG12758.667104E-46.7713936
CCCATTT11800.00274076786.7351361
CTTTGAA15551.6480213E-46.50479031
TTCTTGC14250.00303836756.05861436
CAAATTT13250.0095816645.97255753
CTTTCAT19100.0022833975.29578451
CTTTTCT20850.00120993665.19781541
ATTAAAA24152.9551715E-45.08589551
Produced by FastQC (version 0.11.5)